Characterization of vesicular stomatitis virus populations by tunable resistive pulse sensing

A recent publication by the group of Professor Yin at the University of Wisconsin-Madison highlights the advantages of TRPS for the characterisation of viruses (bullet-shaped, about 100nm size). TRPS results were compared to TEM, the current benchmark for virion measurement, and the author implied that the former technique is more flexible and easier to implement than the latter.

A very important aspect of TRPS is its ability to measure virus particle concentration down to 10-7 particles/mL with the same accuracy as for higher concentrations (linearity study backs this up). This allows to measure with great certainty the total-to-infectious particle ratio for viruses (and bacteria), invaluable information for virologists and vaccine manufacturers.

Please take a look at the publication (http://www.ncbi.nlm.nih.gov/pubmed/25698465 ). This publication is of big interst to all researcher involved in measuring and studying exosomes, which have a similar size range, and which concentration needs to be assessed very precisely.