Single Cell Study - Cellular Structure - Cell Death

Tomocube’s Holotomography (HT) technology provides unprecedented 3D cell imaging experience. HT measures 3D refractive index (RI) tomograms of a live cell, and thus ensures zero stress condition to cells. Furthermore, HT technology enables 3-D dynamics imaging of live cells for a long time-scale.   Main advantages are as follows:

1. Label-free: Cellular membrane and subcellular organelles can clearly be labeled without using exogenous labeling agents, such as fluorescence proteins or dyes. Thus, there are no issues of phototoxicity, photobleaching, and photodamaging.
2. Quantitative imaging capability: HT directly measures cell’s 3D refractive index (RI) maps, which is intrinsic optical properties of materials. The measured RI can be translated into the mass density of a cell and using this information, mass of a cell can also be retrieved.
3. Precise and fast measurements: HT can resolve details as fine as 110 nm (x,y) and 356 nm (z), which is more powerful than the confocal microscopy . HT provides 2D/3D/4D measurements; imaging speed is 2.5 fps for 4D.
4. Cost effective method: Can save the cost for labeling agents. Tomocube’s HT system provides 3D label-free imaging capability with very high spatial resolution at a reasonable price, , compared to the conventional fluorescence methods.

3D holotomography images of various cell lines measured with HT-1S

Tomocube's TomoStudio [Office1] provides unique cell image analysis features. TomoStudio provides powerful features to effectively visualize the 3D RI distributions; users can easily assign peudocolors and transparency to the structures of interest based on RI information. It also enables the automatic segmentation of cell structures, based on the 3D RI tomographic data.

Automatic segmentation of cell structures